Device

Part:BBa_K3024004:Design

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-09-28)


C-protein Characterization Construct (Lysogenic module)

This composite part is composed by the promoter BBa_J23119 (pTet promoter), followed by Tet-Operetaror, the gene that encodes for C protein fused with FLAG-tag (DYKDDDDK epitope) and finally RFP gene as a live-cell reporter. This module can induce the lysogenic cycle of P2 bacteriophage. For more information visit https://2019.igem.org/Team:Stockholm.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 997
    Illegal AgeI site found at 1109
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to meet complexity standards for IDT synthesis.


Source

Assembled from different parts derived from the igem registry, NCBI and uniprot. For details, see the respective basic parts.

References

Christie GE, Calendar R. Bacteriophage P2. Bacteriophage. 2016;6(1):e1145782.